Retrotransposon insertions can initiate colorectal cancer and are associated with poor survival

dc.contributor.affiliationUniversity of Helsinki - Cajuso, Tatiana
dc.contributor.affiliationUniversity of Helsinki - Sulo, Päivi
dc.contributor.affiliationUniversity of Helsinki - Tanskanen, Tomas
dc.contributor.affiliationUniversity of Helsinki - Katainen, Riku
dc.contributor.affiliationUniversity of Helsinki - Taira, Aurora
dc.contributor.affiliationUniversity of Helsinki - Hänninen, Ulrika A
dc.contributor.affiliationUniversity of Helsinki - Kondelin, Johanna
dc.contributor.affiliationUniversity of Helsinki - Forsström, Linda
dc.contributor.affiliationUniversity of Helsinki - Välimäki, Niko
dc.contributor.affiliationUniversity of Helsinki - Aavikko, Mervi
dc.contributor.affiliationUniversity of Helsinki - Kaasinen, Eevi
dc.contributor.affiliationUniversity of Helsinki - Ristimäki, Ari
dc.contributor.affiliationUniversity of Helsinki - Koskensalo, Selja
dc.contributor.affiliationUniversity of Helsinki - Lepistö, Anna
dc.contributor.affiliationUniversity of Helsinki - Renkonen-Sinisalo, Laura
dc.contributor.affiliationUniversity of Helsinki - Seppälä, Toni
dc.contributor.affiliationUniversity of Jyväskylä - Kuopio, Teijo
dc.contributor.affiliationCentral Finland Health Care District - Böhm, Jan
dc.contributor.affiliationJyväskylä Central Hospital - Mecklin, Jucka-Pekka
dc.contributor.affiliationUniversity of Helsinki - Kilpivaara, Outi
dc.contributor.affiliationUniversity of Helsinki - PItkänen, Esa
dc.contributor.affiliationUniversity of Helsinki - Palin, Kimmo
dc.contributor.affiliationUniversity of Helsinki - Aaltonen, Lauri A
dc.contributor.authorCajuso, Tatiana
dc.contributor.authorSulo, Päivi
dc.contributor.authorTanskanen, Tomas
dc.contributor.authorKatainen, Riku
dc.contributor.authorTaira, Aurora
dc.contributor.authorHänninen, Ulrika A
dc.contributor.authorKondelin, Johanna
dc.contributor.authorForsström, Linda
dc.contributor.authorVälimäki, Niko
dc.contributor.authorAavikko, Mervi
dc.contributor.authorKaasinen, Eevi
dc.contributor.authorRistimäki, Ari
dc.contributor.authorKoskensalo, Selja
dc.contributor.authorLepistö, Anna
dc.contributor.authorRenkonen-Sinisalo, Laura
dc.contributor.authorSeppälä, Toni
dc.contributor.authorKuopio, Teijo
dc.contributor.authorBöhm, Jan
dc.contributor.authorMecklin, Jucka-Pekka
dc.contributor.authorKilpivaara, Outi
dc.contributor.authorPItkänen, Esa
dc.contributor.authorPalin, Kimmo
dc.contributor.authorAaltonen, Lauri A
dc.date.accessioned2025-03-24T15:26:42Z
dc.date.issued2019-07-29
dc.date.issued2019-07-29
dc.descriptionThis dataset is related to "Retrotransposon insertions can initiate colorectal cancer and are associated with poor survival" (Cajuso et al.). Abstract: Genomic instability pathways in colorectal cancer (CRC) have been extensively studied, but the role of retrotransposition in colorectal carcinogenesis remains poorly understood. Although retrotransposons are usually repressed, they become active in several human cancers, in particular those of the gastrointestinal tract. Here we characterize retrotransposon insertions in 202 colorectal tumor whole genomes and investigate their associations with molecular and clinical characteristics. We find highly variable retrotransposon activity among tumors and identify recurrent insertions in 15 known cancer genes. In approximately 1% of the cases we identify insertions in APC, likely to be tumor-initiating events. Insertions are positively associated with the CpG island methylator phenotype and the genomic fraction of allelic imbalance. Clinically, high number of insertions is independently associated with poor disease-specific survival. Sample description:  A signed informed consent was obtained for as many human participants as possible. In cases without a signed informed consent, an authorization from the National Supervisory Authority for Welfare and Health (Dnro 421/04/044/06, Dnro 8048/06.01.03.01/2014, Dnro 358/32/300/05, Dnro 1476/06.01.03.01/2012) was obtained as stated in Finnish law. The study has been reviewed by the Ethics Committee of the Hospital district of Helsinki and Uusima (Dnro 133/E8/03, 408/13/03/03/2009). Permission to use patient information was obtained from the National Institute for Health and Welfare (Dnro 53/07/2000, Dnro THL/1071/5.05.00/2011, Dnro THL/151/5.05.00/2017).   RNA sequencing: Total RNA from consecutive cryosections was extracted using RNeasy Mini Kit (Qiagen) from 34 tumors that displayed more than 50% of cancer cell percentage (HE staining of cryosections) and RNA integrity>6 (Agilent RNA 6000, Agilent 2100 Bioanalyzer). Paired-end RNA sequencing was performed on the Illumina Hiseq 2000. RNA-seq data was processed using Kallisto (version 0.43.0) software. Kallisto quantification was executed in paired-end mode and aligned against the Ensembl Human reference transcriptome (GRCh37_79). Quantification results from Kallisto were normalized and aggregated to gene-level utilizing sleuth (version 0.28.1) R package with default filtering settings.  
dc.identifierhttps://doi.org/10.5281/zenodo.3241399
dc.identifier.urihttps://hydatakatalogi-test-24.it.helsinki.fi/handle/123456789/11516
dc.rightsOpen
dc.rights.licensecc-by-4.0
dc.titleRetrotransposon insertions can initiate colorectal cancer and are associated with poor survival
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