Mouse SIRV and simulated data used in the IsoQuant publication
2023-02-06, 2023-02-06
dataset
dataset
dataset
Open
This dataset contain main simulated and SIRV sequencing data used in the IsoQuant publication.
- Reduced mouse GENCODE vM26 annotation: 15% of expressed isoforms removed, expression profile based on real mouse brain sequencing data; also contains GTF with expressed transcripts.
- Mouse PacBio simulated data: 6 million reads generated with IsoSeqSim, 1.4% error rate. Provided as a sorted BAM file, reads were mapped to GRCm39 genome using minimap v2.18.
- Mouse ONT R10.4 simulated data: 30 million read generated with NanoSim, 2.8% error rate. Provided as a sorted BAM file, reads were mapped to GRCm39 genome using minimap v2.18.
- Mouse ONT R9.4 simulated data: 30 million read generated with NanoSim, 15.9% error rate. Provided as a sorted BAM file, reads were mapped to GRCm39 genome using minimap v2.18.
- Lexogen SIRV Set4 sequenced on ONT MinIon with R10.4 chemistry. Provided as a sorted BAM file, reads were mapped to SIRV reference using minimap v2.18. This data was used in the IsoQuant publication.
- Lexogen SIRV Set4 sequenced on ONT MinIon with R9.4 chemistry, filtered >=Q12. Provided as a sorted BAM file, reads were mapped to SIRV reference using minimap v2.18. This data was NOT used in the IsoQuant publication.
- SIRV reference genome and reduced annotation, also available at Lexogen download page.