Data from: Reliable wolf-dog hybrid detection in Europe using a reduced SNP panel developed for non-invasively collected samples

dc.contributor.affiliationUniversity of Oulu - Harmoinen, Jenni
dc.contributor.affiliationGoethe University Frankfurt - von Thaden, Alina
dc.contributor.affiliationUniversity of Oulu - Aspi, Jouni
dc.contributor.affiliationUniversity of Oulu - Kvist, Laura
dc.contributor.affiliationNatural History Museum - Cocchiararo, Berardino
dc.contributor.affiliationGoethe University Frankfurt - Jarausch, Anne
dc.contributor.affiliationAssociation for the Conservation of Biological Diversity, Romania* - Gazzola, Andrea
dc.contributor.affiliationUniversity of Bucharest - Sin, Teodora
dc.contributor.affiliationUniversity of Helsinki - Lohi, Hannes
dc.contributor.affiliationUniversity of Helsinki - Hytönen, Marjo
dc.contributor.affiliationNatural Resources Institute Finland - Kojola, Ilpo
dc.contributor.affiliationUniversity of Ljubljana - Stronen, Astrid Vik
dc.contributor.affiliationIstituto Superiore per la Protezione e la Ricerca Ambientale - Caniglia, Romolo
dc.contributor.affiliationIstituto Superiore per la Protezione e la Ricerca Ambientale - Mattucci, Federica
dc.contributor.affiliationWorld Wide Fund for Nature - Galaverni, Marco
dc.contributor.affiliationUniversity of Porto - Godinho, Raquel
dc.contributor.affiliationUniversity of the Basque Country - Ruiz-González, Aritz
dc.contributor.affiliationUniversity of Bologna - Randi, Ettore
dc.contributor.affiliationEuropean Bioinformatics Institute - Muñoz-Fuentes, Violeta
dc.contributor.affiliationNatural History Museum - Nowak, Carsten
dc.contributor.authorHarmoinen, Jenni
dc.contributor.authorvon Thaden, Alina
dc.contributor.authorAspi, Jouni
dc.contributor.authorKvist, Laura
dc.contributor.authorCocchiararo, Berardino
dc.contributor.authorJarausch, Anne
dc.contributor.authorGazzola, Andrea
dc.contributor.authorSin, Teodora
dc.contributor.authorLohi, Hannes
dc.contributor.authorHytönen, Marjo
dc.contributor.authorKojola, Ilpo
dc.contributor.authorStronen, Astrid Vik
dc.contributor.authorCaniglia, Romolo
dc.contributor.authorMattucci, Federica
dc.contributor.authorGalaverni, Marco
dc.contributor.authorGodinho, Raquel
dc.contributor.authorRuiz-González, Aritz
dc.contributor.authorRandi, Ettore
dc.contributor.authorMuñoz-Fuentes, Violeta
dc.contributor.authorNowak, Carsten
dc.date.accessioned2025-03-24T15:21:19Z
dc.date.issued2021-05-10
dc.date.issued2021-05-10
dc.descriptionBackground: Understanding the processes that lead to hybridization of wolves and dogs is of scientific and management importance, particularly over large geographical scales, as wolves can disperse great distances. However, a method to efficiently detect hybrids in routine wolf monitoring is lacking. Microsatellites offer only limited resolution due to the low number of markers showing distinctive allele frequencies between wolves and dogs. Moreover, calibration across laboratories is time-consuming and costly. In this study, we selected a panel of 96 ancestry informative markers for wolves and dogs, derived from the Illumina CanineHD Whole-Genome BeadChip (174K). We designed very short amplicons for genotyping on a microfluidic array, thus making the method suitable also for non-invasively collected samples. Results: Genotypes based on 93 SNPs from wolves sampled throughout Europe, purebred and non-pedigree dogs, and suspected hybrids showed that the new panel accurately identifies parental individuals, first-generation hybrids and first-generation backcrosses to wolves, while second- and third-generation backcrosses to wolves were identified as advanced hybrids in almost all cases. Our results support the hybrid identity of suspect individuals and the non-hybrid status of individuals regarded as wolves. We also show the adequacy of these markers to assess hybridization at a European-wide scale and the importance of including samples from reference populations. Conclusions: We showed that the proposed SNP panel is an efficient tool for detecting hybrids up to the third-generation backcrosses to wolves across Europe. Notably, the proposed genotyping method is suitable for a variety of samples, including non-invasive and museum samples, making this panel useful for wolf-dog hybrid assessments and wolf monitoring at both continental and different temporal scales.
dc.identifierhttps://doi.org/10.5061/dryad.76hdr7stk
dc.identifier.urihttps://hydatakatalogi-test-24.it.helsinki.fi/handle/123456789/10838
dc.rightsOpen
dc.rights.licensecc-zero
dc.titleData from: Reliable wolf-dog hybrid detection in Europe using a reduced SNP panel developed for non-invasively collected samples
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