Data from: Ornithine Decarboxylase Antizyme Inhibitor 2 (AZIN2) is a signature of secretory phenotype and independent predictor of adverse prognosis in colorectal cancer

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dc.contributor.affiliationHelsinki University Central Hospital - Kaprio, Tuomas
dc.contributor.affiliationUniversity of Helsinki - Rasila, Tina
dc.contributor.affiliationUniversity of Helsinki - Hagström, Jaana
dc.contributor.affiliationHelsinki University Central Hospital - Mustonen, Harri
dc.contributor.affiliationHelsinki University Central Hospital - Haglund, Caj
dc.contributor.affiliationUniversity of Helsinki - Andersson, Leif C.
dc.contributor.authorKaprio, Tuomas
dc.contributor.authorRasila, Tina
dc.contributor.authorHagström, Jaana
dc.contributor.authorMustonen, Harri
dc.contributor.authorHaglund, Caj
dc.contributor.authorAndersson, Leif C.
dc.date.accessioned2025-03-24T15:14:55Z
dc.date.issued2019-02-19
dc.date.issued2019-02-19
dc.descriptionOrnithine decarboxylase (ODC) is the rate-limiting enzyme of polyamine synthesis. The two ODC antizyme inhibitors (AZIN1) and (AZIN2) are regulators of the catalytic activity of ODC. While AZIN1 is a regulator of cell proliferation, AZIN2 is involved in intracellular vesicle transport and secretion. There are no previous reports on the impact of AZIN2 expression in human cancer. We applied immunohistochemistry with antibodies to human AZIN2 on tissue micro- arrays of colorectal cancers (CRC) from 840 patients with a median follow-up of 5.1 years (range 0-25.8). The 5-year disease-specific survival rate was 58.9% (95% Cl 55.0-62.8%). High AZIN2 expression was associated with mucinous histology (p=0.002) and location in the right hemicolon (p=0.021). We found no association with age, gender, stage, or histological tumor grade. High tumor expression of AZIN2 predicted an unfavorable prognosis (p<0.0001, log-rank test), compared to low AZIN2 expression. Cox multivariable analysis identified AZIN2 as an independent factor of an unfavorable prognosis in CRC. The strongest AZIN2 expression was seen in invasive tumor cells having morphological features of epithelial-mesenchymal transition (EMT). Induction of EMT in HT-29 CRC cells lead to upregulated expression of endogenous AZIN2. Given that AZIN2 is a regulator of vesicle transport and secretion, we overexpressed human AZIN2 cDNA in T84 CRC cells, and found strongly enhanced accumulation of CD63-positive exosomes in the culture medium. These findings indicate that AZIN2 expression is a signature of EMT-associated secretory phenotype that is linked to an adverse prognosis in CRC.
dc.identifierhttps://doi.org/10.5061/dryad.h3t3qd3
dc.identifier.urihttps://hydatakatalogi-test-24.it.helsinki.fi/handle/123456789/9416
dc.rightsOpen
dc.rights.licensecc-zero
dc.titleData from: Ornithine Decarboxylase Antizyme Inhibitor 2 (AZIN2) is a signature of secretory phenotype and independent predictor of adverse prognosis in colorectal cancer
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