Data from: Carrion fly-derived DNA metabarcoding is an effective tool for mammal surveys: evidence from a known tropical mammal community

dc.contributor.affiliationSmithsonian Tropical Research Institute - Rodgers, Torrey W.
dc.contributor.affiliationUniversity of Groningen - Xu, Charles C. Y.
dc.contributor.affiliationMontclair State University - Giacalone, Jacalyn
dc.contributor.affiliationSmithsonian Tropical Research Institute - Kapheim, Karen M.
dc.contributor.affiliationSmithsonian Tropical Research Institute - Saltonstall, Kristin
dc.contributor.affiliationSmithsonian Tropical Research Institute - Vargas, Marta
dc.contributor.affiliationKunming Institute of Zoology - Yu, Douglas W.
dc.contributor.affiliationUniversity of Helsinki - Somervuo, Panu
dc.contributor.affiliationSmithsonian Tropical Research Institute - McMillan, W. Owen
dc.contributor.affiliationWageningen University & Research - Jansen, Patrick A.
dc.contributor.authorRodgers, Torrey W.
dc.contributor.authorXu, Charles C. Y.
dc.contributor.authorGiacalone, Jacalyn
dc.contributor.authorKapheim, Karen M.
dc.contributor.authorSaltonstall, Kristin
dc.contributor.authorVargas, Marta
dc.contributor.authorYu, Douglas W.
dc.contributor.authorSomervuo, Panu
dc.contributor.authorMcMillan, W. Owen
dc.contributor.authorJansen, Patrick A.
dc.coverage.spatialBarro Colorado Island
dc.coverage.spatialPanama
dc.date.accessioned2025-03-24T15:21:20Z
dc.date.issued2017-07-27
dc.date.issued2017-07-27
dc.descriptionMetabarcoding of vertebrate DNA derived from carrion flies has been proposed as a promising tool for biodiversity monitoring. To evaluate its efficacy, we conducted metabarcoding surveys of carrion flies on Barro Colorado Island (BCI), Panama, which has a well-known mammal community, and compared our results against diurnal transect counts and camera-trapping. We collected 1084 flies in 29 sampling days, conducted metabarcoding with mammal-specific (16S) and vertebrate-specific (12S) primers, and sequenced amplicons on Illumina MiSeq. For taxonomic assignment, we compared BLAST with the new program PROTAX, and we found that PROTAX improved species identifications. We detected 20 mammal, four bird, and one lizard species from carrion fly metabarcoding, all but one of which are known from BCI. Fly metabarcoding detected more mammal species than concurrent transect counts (29 sampling days, 13 species) and concurrent camera-trapping (84 sampling days, 17 species), and detected 67% of the number of mammal species documented by eight years of transect counts and camera-trapping combined, although fly metabarcoding missed several abundant species. This study demonstrates that carrion fly metabarcoding is a powerful tool for mammal biodiversity surveys, and has the potential to detect a broader range of species than more commonly used methods.
dc.identifierhttps://doi.org/10.5061/dryad.bj5k0
dc.identifier.urihttps://hydatakatalogi-test-24.it.helsinki.fi/handle/123456789/10848
dc.rightsOpen
dc.rights.licensecc-zero
dc.subjectBarro Colorado Island
dc.subjecttransect counts
dc.titleData from: Carrion fly-derived DNA metabarcoding is an effective tool for mammal surveys: evidence from a known tropical mammal community
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