Data from: Intestinal Ralstonia pickettii augments glucose intolerance in obesity

dc.contributor.affiliationUniversity of Gothenburg - Kovatcheva-Datchary, Petia
dc.contributor.affiliationAcademic Medical Center - Herrema, Hilde
dc.contributor.affiliationWageningen University & Research - Belzer, Clara
dc.contributor.affiliationAcademic Medical Center - Witjes, Julia J.
dc.contributor.affiliationAcademic Medical Center - Vrieze, Anne
dc.contributor.affiliationAcademic Medical Center - Chaplin, Alice
dc.contributor.affiliationWageningen University & Research - Aalvink, Steven
dc.contributor.affiliationUniversity of Gothenburg - Bergstrom, Goran
dc.contributor.affiliationAcademic Medical Center - Holleman, Frits
dc.contributor.affiliationUniversity of Copenhagen - Backhed, Fredrik
dc.contributor.affiliationUniversity of Gothenburg - Nieuwdorp, Max
dc.contributor.affiliationAcademic Medical Center - Udayappan, Shanthadevi D.
dc.contributor.affiliationAcademic Medical Center - Bakker, Guido J.
dc.contributor.affiliationAcademic Medical Center - Havik, Stefan R.
dc.contributor.affiliationAcademic Medical Center - Bouter, Kristien E.
dc.contributor.affiliationAcademic Medical Center - Dallinga-Thie, Geesje M.
dc.contributor.affiliationAcademic Medical Center - Stroes, Erik S. G.
dc.contributor.affiliationUniversity of Groningen - Groen, Albert K.
dc.contributor.affiliationWageningen University & Research - Heilig, Hans G. H. J.
dc.contributor.affiliationUniversity of Helsinki - de Vos, Willem M.
dc.contributor.affiliationAcademic Medical Center - de Sonnaville, Noor
dc.contributor.affiliationAcademic Medical Center - Hoekstra, Joost B. L.
dc.contributor.affiliationVU University Medical Center - van Raalte, Daniel H.
dc.contributor.authorUdayappan, Shanthadevi
dc.contributor.authorKovatcheva-Datchary, Petia
dc.contributor.authorBakker, Guido
dc.contributor.authorHavik, Stefan
dc.contributor.authorHerrema, Hilde
dc.contributor.authorCani, Patrice
dc.contributor.authorBouter, Kristien
dc.contributor.authorBelzer, Clara
dc.contributor.authorWitjes, Julia J.
dc.contributor.authorVrieze, Anne
dc.contributor.authorde Sonnaville, Eleonore Susanne Victoria
dc.contributor.authorChaplin, Alice
dc.contributor.authorvan Raalte, Daniel
dc.contributor.authorAalvink, Steven
dc.contributor.authorDallinga-Thie, Geesje
dc.contributor.authorHeilig, Hans
dc.contributor.authorBergstrom, Goran
dc.contributor.authorvan der Meij, Suzan
dc.contributor.authorVan Wagensveld, Bart
dc.contributor.authorHoekstra, Joost
dc.contributor.authorHolleman, Frits
dc.contributor.authorStroes, Erik
dc.contributor.authorGroen, Albert
dc.contributor.authorBackhed, Fredrik
dc.contributor.authorde Vos, Willem
dc.contributor.authorNieuwdorp, Max
dc.contributor.authorUdayappan, Shanthadevi D.
dc.contributor.authorBakker, Guido J.
dc.contributor.authorHavik, Stefan R.
dc.contributor.authorBouter, Kristien E.
dc.contributor.authorDallinga-Thie, Geesje M.
dc.contributor.authorStroes, Erik S. G.
dc.contributor.authorGroen, Albert K.
dc.contributor.authorHeilig, Hans G. H. J.
dc.contributor.authorde Vos, Willem M.
dc.contributor.authorde Sonnaville, Noor
dc.contributor.authorHoekstra, Joost B. L.
dc.contributor.authorvan Raalte, Daniel H.
dc.date.accessioned2025-03-24T15:23:19Z
dc.date.issued2018-10-17
dc.date.issued2018-10-17
dc.descriptionAn altered intestinal microbiota composition has been implicated in the pathogenesis of metabolic disease including obesity and type 2 diabetes mellitus (T2DM). Low grade inflammation, potentially initiated by the intestinal microbiota, has been suggested to be a driving force in the development of insulin resistance in obesity. Here, we report that bacterial DNA is present in mesenteric adipose tissue of obese but otherwise healthy human subjects. Pyrosequencing of bacterial 16S rRNA genes revealed that DNA from the Gram-negative species Ralstonia was most prevalent. Interestingly, fecal abundance of Ralstonia pickettii was increased in obese subjects with pre-diabetes and T2DM. To assess if R. pickettii was causally involved in development of obesity and T2DM, we performed a proof-of-concept study in diet-induced obese (DIO) mice. Compared to vehicle-treated control mice, R. pickettii-treated DIO mice had reduced glucose tolerance. In addition, circulating levels of endotoxin were increased in R. pickettii-treated mice. In conclusion, this study suggests that intestinal Ralstonia is increased in obese human subjects with T2DM and reciprocally worsens glucose tolerance in DIO mice.
dc.identifierhttps://doi.org/10.5061/dryad.q5s51
dc.identifier.urihttps://hydatakatalogi-test-24.it.helsinki.fi/handle/123456789/11103
dc.rightsOpen
dc.rights.licensecc-zero
dc.subjectglucose intolerance
dc.subjectRalstonia pickettii
dc.subjectObesity
dc.titleData from: Intestinal Ralstonia pickettii augments glucose intolerance in obesity
dc.typedataset
dc.typedataset
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