cc-by-4.0Ilya, BelevichJokitalo, Eija2025-03-242023-10-262023-10-26https://hydatakatalogi-test-24.it.helsinki.fi/handle/123456789/9283This submission includes ground truth datasets that were used to segment the nuclear envelope (NE), mitochondria, endoplasmic reticulum (ER) and Golgi from a human bone osteosarcoma epithelial cell (U2-OS) imaged using focused-ion beam scanning electron microscopy (FIB-SEM).The full FIB-SEM dataset is deposited to EMPIAR (https://www.ebi.ac.uk/empiar, EMPIAR-11746). Opendataset